Undergraduate
Graduate
Alumni
Information
Search

(Main banner image: Research Group 2012: “Team Dicty” From left to right: Emily Reed, Caitlin Pozmanter, Susan Parrish, and Tiara Tirasawasdichai)

Dr. Susan Parrish

Research Group 2012: “Team Dicty” From left to right: Emily Reed, Caitlin Pozmanter, Susan Parrish, and Tiara Tirasawasdichai

Research Interests

Molecular Biology Research:

My laboratory studies mechanisms that modulate RNA levels to influence how genes are expressed, particularly during infection and development. My students and I have biochemically characterized several Nudix mRNA decapping enzymes encoded by the nucleocytoplasmic large DNA viruses, including poxviruses, asfarviruses, and the giant Mimiviruses. These enzymes are thought to destroy competing host cellular mRNAs, thereby allowing the viruses preferential access to the cellular machinery.

Currently in the lab, we are examining the role of Nudix mRNA decapping enzymes during the development of the social amoeba, Dictyostelium discoideum. D. discoideum normally lives as a single-celled amoeba; however, when starved for nutrients, many cells come together to form a multicellular fruiting body with specialized cell types. A subset of these cells will become spore cells, which give rise to the single-celled amoebae when food is available again. This transition to the multicellular life stage, termed development, requires significant changes in gene expression. We are interested in determining whether the Nudix mRNA decapping enzymes are required for the changes in gene expression that occur during development of D. discoideum.

Genomics Research:

I am a member of the Genomics Education Partnership (GEP) http://gep.wustl.edu/, a collaborative educational initiative that provides undergraduate students with the opportunity to participate in original Genomics research projects in a classroom laboratory setting. Students annotate raw DNA sequence data obtained from several different species of Drosophila, more commonly known as the fruit fly. Genome annotation provides meaning to the string of letters of DNA sequence, identifying features such as genes and their regulatory regions. In addition to decoding the DNA sequence, these annotation projects are used to answer broader biological questions, such as the evolutionary relationships between Drosophila species and the possible link between sequence information and higher order chromatin structure. After completion, the annotation projects are submitted to scientific databases for public use and the results are published in scientific journals. We are now preparing a manuscript for publication that will contain 34 McDaniel College students as co-authors.

Courses Taught

  • FYS 1109 Our Unseen Enemies: Emerging Viruses
  • BIO 1111 Principles of Biology
  • BIO 1118 Human Biology
  • BIO 2208 Molecular Biology Lecture & Laboratory
  • BIO 3311 Advanced Genetics-Molecular
  • BIO 3312 Genomics Lecture & Laboratory

Publications*

* Indicates McDaniel College student author.

- C. Shaffer, C. Alvarez, A. Bednarski, D. Dunbar, A. Goodman, C. Reinke, A. Rosenwald, M. Wolyniak, C. Bailey, D. Barnard, C. Bazinet, D. Beach, J. Bedard, S.Bhalla, J. Braverman, M. Burg, V. Chandrasekaran, H. Chung, K. Clase, R. DeJong, J. DiAngelo, C. Du, T. Eckdahl, H. Eisler, J. Emerson, A. Frary, D. Frohlich, Y. Gosser, S. Govind, A. Haberman, A. Hark, C. Hauser, A. Hoogewerf, L. Hoopes,C. Howell, D. Johnson, C. Jones, L. Kadlec, M. Kaehler, S. Silver Key, A. Kleinschmit, N. Kokan, O. Kopp, Gary Kuleck, J. Leatherman, J. Lopilato, C. MacKinnon, J. Martinez-Cruzado, G. McNeil, S. Mel, H. Mistry, A. Nagengast, P. Overvoorde, D. Paetkau, S. Parrish, C. Peterson, M. Preuss, L. Reed, D. Revie, S. Robic, J. Roecklein-Canfield, M. Rubin, K. Saville, S. Schroeder, K. Sharif, M. Shaw,G. Skuse, C. Smith, M. Smith, S. Smith, E. Spana, M. Spratt, A. Sreenivasan, J. Stamm, P. Szauter, J. Thompson, M. Wawersik, J. Youngblom, L. Zhou, E. Mardis, J. Buhler, W. Leung,D. Lopatto, and S. Elgin. 2014. A Course-Based Research Experience: How Benefits Change with Increased Investment in Instructional Time. CBE-Life Sciences Education. In press.

- R. Alexander, C. Bailey, J. Bedard, B. Brown, M. Burg, S. Celniker, S. Elgin, K. Hogan, C. Jones, T. Kawil, C. Kelly, N. Kokan, R. Lowdon, M. McCarthy, I. McDaniel, P. Meneely, P. Park, S. Parrish, J. Roecklein-Canfield, V. Reinke, C. Silver Key, G. Shemer, M. Smith, B. Starr, B. Waterston, and L. Zahn. 2012. ModENCODE: Model Organism ENCyclopedia of DNA Elements Educational Supplement. Science Magazine. Website: http://modencode.sciencemag.org/

- C. Shaffer, C. Alvarez, C. Bailey, D. Barnard, S. Bhalla, C. Chandrasekaran, V. Chandrasekaran, H. Chung, D. Dorer, C. Du, T. Eckdahl, J. Poet, D. Frohlich, A. Goodman, Y. Gosser, C. Hauser,L. Hoopes, D. Johnson, C. Jones, M. Kaehler, N. Kokan, O. Kopp, G. Kuleck, G. McNeil, R. Moss, J. Myka, A. Nagengast, R. Morris, P. Overvoorde, E. Shoop, S. Parrish, K. Reed, E. Regisford, D. Revie, A. Rosenwald, K. Saville, S. Schroeder, M. Shaw, G. Skuse, C. Smith, M. Smith, E. Spana, M. Spratt, J. Stamm, J. Thompson, M. Wawersik, B. Wilson, J. Youngblom, W. Leung, J. Buhler, E. Mardis, D. Lopatto, and S. Elgin. 2010. The Genomics Education Partnership: Successful integration of research into laboratory classes at a diverse group of undergraduate institutions. CBE-Life Sciences Education. 9, 55-69.

- S. Parrish, M. Hurchalla*, S. Liu, and B. Moss. 2009. The African Swine Fever Virus (ASFV) g5R protein possesses mRNA decapping activity. Virology. 393, 177-182.

- S. Parrish and B. Moss. 2007. Characterization of a second vaccinia virus mRNA decapping enzyme conserved in poxviruses. Journal of Virology. 81, 12973-12978.

- S. Parrish, W. Resch, and B. Moss. 2007. Vaccinia virus D10 protein has mRNA decapping activity, providing a mechanism for control of host and viral gene expression. Proceedings of the National Academy of Sciences. 104, 2139-2144.

- S. Parrish and B. Moss. 2006. Characterization of a vaccinia virus mutant with a deletion of the D10R gene encoding a putative negative regulator of gene expression. Journal of Virology. 80, 53-561.

- F. Simmer, M. Tijsterman, S. Parrish, S. Koushika, M. Nonet, A. Fire, J. Ahringer, and R. Plasterk. 2002. Loss of the putative RNA directed RNA polymerase RRF-3 makes C. elegans hypersensitive to RNAi. Current Biology. 12, 1317-1319.

- T. Sijen, J. Fleenor, F. Simmer, K. Thijssen, S. Parrish, L. Timmons, R. Plasterk, and A. Fire. 2001. On the role of RNA amplification in dsRNA-triggered gene silencing. Cell. 107, 465-476.

- S. Parrish and A. Fire. 2001. Distinct roles for RDE-1 and RDE-4 during RNA interference in Caenorhabditis elegans. RNA. 7, 1397-1402.

- N. Caplen, S. Parrish, F. Imani, A. Fire, and R. Morgan. 2001. Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems. Proceedings of the National Academy of Sciences. 98, 9742-9747.

- A. Grishok, A. Pasquinelli, D. Conte, N. Li, S. Parrish, I. Ha, D. Baillie, A. Fire, G.Ruvkun, and C. Mello. 2001. Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing. Cell. 106, 23-34.

- S. Parrish, J. Fleenor, S. Xu, C. Mello, and A. Fire. 2000. Functional anatomy of a dsRNA trigger: differential requirement for the two trigger strands in RNA interference. Molecular Cell. 6, 1077-1087.

- A. Agarwal, S. Parrish, and D. Blumberg. 1999. Ribosomal protein gene expression is cell type specific during development in Dictyostelium discoideum. Differentiation. 65, 73-88.

Scientific Conference Presentations*

* Indicates McDaniel College student author.

- K. Bickford*, C. O'Keeffe*, M. Nelson*, and S. Parrish. 2013. Characterization of Nudix mRNA decapping enzymes in Dictyostelium discoideum. Annual International Dictyostelium Conference, Asheville, NC. Poster.

- W. Neutzling*, M. Jones*, and S. Parrish. 2013. A comparative genomic analysis of the Drosophila dot chromosome. 54th Annual Drosophila Research Conference. Washington, D.C. Poster.

- C. Marques Dos Santos Vieira*, T. Tirasawasdichai*, and S. Parrish. 2013. Annotation of Fosmid 60 of Drosophila erecta and of DMAC 47 of Drosophila mojavensis as control sequences in the comparative genomic analysis of the Drosophila dot chromosome. 54th Annual Drosophila Research Conference. Washington, D.C. Poster.

- S. Elgin, M Burg, J. DiAngelo, A. Haberman, C. Jones, L. Kadlec, C. Silver Key, J. Leatherman, G. McNeil, A. Nagengast, D. Paetkau, S. Parrish, L. Reed, S. Smith, L. Zhou, W. Leung, C. Shaffer, and D. Lopatto. 2013. The Genomics Education Partnership (GEP): Bringing Genomics Research into Undergraduate Classrooms. 54th Annual Drosophila Research Conference. Washington, D.C. Poster.

- C. O’Keeffe* and S. Parrish. 2013. Creating a genetic knock-out in the social amoeba Dictyostelium discoideum. The Maryland 2013 Collegiate Honors Council. Germantown, MD. Poster.

- C. O’Keeffe* and S. Parrish. 2012. Creation of a genetic knock-out of the Dictyostelium discoideum DDB_G0278957 gene, encoding a putative mRNA Nudix decapping enzyme. 15th Annual Undergraduate Research symposium in the Chemical and Biological Sciences. University of Maryland Baltimore County, Baltimore, MD. Poster.

- K. Bickford* and S. Parrish. 2012. Transformation and gene knock-out of the putative mRNA decapping enzyme DDB_G0283315 in Dictyostelium discoideum. 15th Annual Undergraduate Research symposium in the Chemical and Biological Sciences. University of Maryland Baltimore County, Baltimore, MD. Poster.

- C. Pozmanter*, K. Moran*, and S. Parrish. 2011. Cloning and biochemical characterization of Dictyostelium discoideum DDB_GO278957, a putative mRNA decapping enzyme. 14th Annual Undergraduate Research symposium in the Chemical and Biological Sciences. University of Maryland Baltimore County, Baltimore, MD. Poster.

- S. Parrish and G. Kago*. 2011. The Mimivirus L375 protein possesses intrinsic mRNA decapping activity. American Society for Biochemistry and Molecular Biology Annual Meeting. Washington, D.C. Poster.

- A. Hark, C. Bailey, S. Parrish, C. Shaffer, W. Leung, and S. Elgin. 2011. Undergraduate research in the Genomics Education Partnership: a comparative genomics project exploring genome organization and chromatin structure in Drosophila. American Society for Biochemistry and Molecular Biology Annual Meeting. Washington, D.C. Poster.

- K. Vondy* and S. Parrish. 2010. mRNA Decapping by the Mimivirus L534 protein. 13th Annual Undergraduate Research symposium in the Chemical and Biological Sciences. University of Maryland Baltimore County, Baltimore, MD. Poster.

- J. Bryant* and S. Parrish. 2010. Comparative genomic analysis of the Drosophila dot chromosome. 51st Annual Drosophila Research Conference. Washington, D.C. Poster.

- J. Langley*, M. Shelton*, and S. Parrish. 2010. Annotation of a 49 kb segment of the Drosophila erecta dot chromosome. 51st Annual Drosophila Research Conference. Washington, D.C. Poster.

- B. Yarrington*, S. Hirsch*, and S. Parrish. 2010. The Drosophila erecta dot chromosome: A collaborative annotation. 51st Annual Drosophila Research Conference. Washington, D.C. Poster.

- S. Elgin, M. Burg, B. Chandrasekaran, H. Chung, D. Dorer, D. Johnson, C. Jones, L. Kadlec, S. Silver Key, G. McNeil, A. Nagengast, D. Paetkau, S. Parrish, S. Smith, J. Stamm, M. Wawersik, L. Zhou, W. Leung, C. Shaffer, and D. Lopatto. 2010. The Genomics Education Partnership: Bringing Genomics into the undergraduate curriculum in diverse settings. 51st Annual Drosophila Research Conference. Washington, D.C. Poster.

- E. Lemmon* and S. Parrish. 2009. Annotation of the Drosophila erecta dot chromosome. 1st RECOMB BE Conference. University of California, San Diego, La Jolla, CA. Poster.

- A. Goodman, S. Bhalla, C Hauser, G. Kuleck, S. Parrish, W. Leung, D Lopatto, and S. Elgin. 2009. The Genomics Education Partnership: Integration of DNA sequence finishing and annotation projects into undergraduate curriculum. 1st RECOMB BE Conference. University of California, San Diego, La Jolla, CA. Poster.

- G. Kago* and S. Parrish. 2009. The Mimivirus L375 protein possesses intrinsic mRNA decapping activity. 12th Annual Undergraduate Research symposium in the Chemical and Biological Sciences. University of Maryland Baltimore County, Baltimore, MD. Poster.

- McDaniel College student Grace Kago won first place for her poster in the Biochemistry and Molecular Biology session.

- M. Hurchalla* and S. Parrish. 2008. The African Swine Fever Virus (ASFV) g5R protein possesses mRNA decapping activity. 11th Annual Undergraduate Research symposium in the Chemical and Biological Sciences. University of Maryland Baltimore County, Baltimore, MD. Poster.

- McDaniel College student Megan Hurchalla won first place for her poster in the Biochemistry and Molecular Biology session.

- S. Parrish, M. Hurchalla*, and B. Moss. 2008. The African Swine Fever Virus (ASFV) g5R protein possesses mRNA decapping activity. The 2008 National Institutes of Health Research Festival. Bethesda, MD. Poster.

- S. Parrish and B. Moss. 2007. The vaccinia virus D10 protein possesses mRNA decapping activity, providing a mechanism to control cellular and viral gene expression. 26th annual American Society for Virology Meeting. Oregon State University, Corvallis, OR. Talk.

- S. Parrish and B. Moss. 2005. Analysis of the roles of D9 and D10 proteins in vaccinia virus replication. 24th annual American Society for Virology Meeting. University of Pennsylvania, PA. Talk.

- S. Parrish and B. Moss. 2004. Construction and analysis of D9 and D10 deletion mutants of vaccinia virus. XVth International Poxvirus and Iridovirus Conference. Oxford, England. Poster.

- S. Parrish and A. Fire. 2001. Exploring the molecular mechanism of RNA interference (RNAi). Gordon Research Conference: Viruses & Cells, Tilton, New Hampshire. Talk.

- S. Parrish and A. Fire. 2000. Following the in vivo fate of the double-stranded trigger RNA during RNA-mediated interference (RNAi). East Coast C. elegans Meeting, Atlanta, GA. Poster.

- S. Parrish and A. Fire. 1999. Investigating the fates of trigger RNAs and target transcripts in RNA-mediated interference. International C. elegans Meeting, Madison, WI. Poster.

- A. Fire, J. Fleenor, and S. Parrish. 1999. Structural Requirements for the trigger in RNA-mediated interference. International C. elegans Meeting, Madison, WI. Poster.

- S. Parrish and A. Fire. 1998. A screen for mutants with altered RNAi. East Coast C. elegans Meeting, Boston, MA. Poster.

 
Terror Trot & Block and Roll at Gill Center
November 1, 2014, 12:00 am
Spring Undergraduate Registration
November 3, 2014, 12:00 am
BINGO with GTP at GLAR Dining Hall PORCH
November 4, 2014, 7:00 pm
McDaniel Free Press Weekly Meeting
November 5, 2014, 8:30 pm
Fall Open House at WMC Alumni Hall
November 9, 2014, 11:00 am
Student Solo Recital at McDaniel Lounge
November 14, 2014, 7:30 pm